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Brightfield Microscopy, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in <t>brightfield</t> (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).
Brightfield Microscopy Keyence, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in <t>brightfield</t> (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).
Light Microscopy Zeiss Axioimager M1 Epifluorescence And Brightfield Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/light microscopy zeiss axioimager m1 epifluorescence and brightfield microscope/product/Carl Zeiss
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light microscopy zeiss axioimager m1 epifluorescence and brightfield microscope - by Bioz Stars, 2026-03
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brightfield microscopy  (Carl Zeiss)
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Carl Zeiss brightfield microscopy
( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in <t>brightfield</t> (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).
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Average 90 stars, based on 1 article reviews
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( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in <t>brightfield</t> (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).
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( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in <t>brightfield</t> (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).
Inverted Microscope For Brightfield Microscopy Carl Zeiss Model Axiovert 200, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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upright brightfield microscopy system microscope  (Olympus)
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Olympus upright brightfield microscopy system microscope
( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in <t>brightfield</t> (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).
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( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in brightfield (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).

Journal: eLife

Article Title: A single cysteine residue in vimentin regulates long non-coding RNA XIST to suppress epithelial–mesenchymal transition and stemness in breast cancer

doi: 10.7554/eLife.104191

Figure Lengend Snippet: ( A ) Morphology of MCF-7-expressing WT-VIM and C328S-VIM in brightfield (scale bar = 100 µm). ( B ) Morphology of WT and C328S cells stained with CellMask deep red dye. Images were captured by INCA 2200 and analysed using INCarta software (scale bar = 50 µm). ( C ) Differences in nuclear area, nuclei form factor, nuclear major axis, nuclear minor axis, cell area, cell diameter, cell perimeter, cell minor axis, cell major axis, and cell form factor between the two cell lines. ( D ) Significant reduction in nuclear compactness and nuclei/cell area between the two cell lines. Proliferation rate was compared between WT and C328S cells by ( E, F ) colony, ( G ) MTT, and ( H ) CyQUANT assays. ( I ) CyQUANT cell adhesion assay was performed to compare the cell adhesion between WT and C328S cells without substrate, and ( J ) with the addition of laminin, fibronectin, and collagen, separately. ( K ) Chemotactic migration of the WT and C328S cells through 8.0 µm culture inserts. The cells were fixed and stained with 0.1% (w/v) crystal violet before imaging. ( L ) The cells on the outer surface of the inserts were counted and compared between WT and C328S. ( M ) Chemotactic invasion in WT and C328S cells through 8.0 µm culture inserts coated with Matrigel. The cells were fixed and stained with 0.1% (w/v) crystal violet. ( N ) Total number of cells invaded on the outer surface of the inserts was counted. Statistical analyses: n = 3, error bars = ± SEM, Student’s t -test was used to calculate p values using Microsoft Excel and are given by asterisks (*p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001).

Article Snippet: Colour development was achieved using 3, 3’-diaminobenzidine (DAB) substrate, and slides were lightly counterstained with Harris haematoxylin, dehydrated, mounted in Permount, and imaged by brightfield microscopy (Keyence Corporation, Itasca, IL).

Techniques: Expressing, Staining, Software, CyQUANT Assay, Cell Adhesion Assay, Migration, Imaging